Epstein-Barr virus in nasal T-cell lymphomas in patients with lethal midline granuloma

Lancet. 1990 Jan 20;335(8682):128-30. doi: 10.1016/0140-6736(90)90002-m.


Five cases of lethal midline granuloma were identified histologically and phenotypically as peripheral T-cell lymphomas. Epstein-Barr virus (EBV) DNA was detected in the nasal tumour biopsy specimens by Southern blotting and in-vitro hybridisation with simultaneous detection of EBV-determined nuclear antigen (EBNA) and T-cell surface markers by two-colour immunofluorescence. Further immunofluorescence and northern blotting revealed that EBNA2 gene and also latent membrane protein gene were expressed in the nasal tumour cells. The patients had high titres of antibodies to EBV. These findings suggest that lethal midline granuloma is causally associated with EBV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Viral / analysis
  • Antigens, Viral / analysis
  • Capsid Proteins*
  • DNA, Viral / analysis
  • Epstein-Barr Virus Nuclear Antigens
  • Female
  • Fluorescent Antibody Technique
  • Genes, Viral
  • Granuloma, Lethal Midline / microbiology*
  • Granuloma, Lethal Midline / pathology
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / isolation & purification*
  • Humans
  • Immunoglobulin A / analysis
  • Immunoglobulin G / analysis
  • Lymphoma / microbiology*
  • Lymphoma / pathology
  • Male
  • Nucleic Acid Hybridization
  • Phenotype
  • T-Lymphocytes


  • Antibodies, Viral
  • Antigens, Viral
  • Capsid Proteins
  • DNA, Viral
  • Epstein-Barr Virus Nuclear Antigens
  • Epstein-Barr viral capsid antigen
  • Epstein-Barr virus early antigen
  • Immunoglobulin A
  • Immunoglobulin G