Preparation, characterization, and antiproliferative activities of the Se-containing polysaccharide SeGLP-2B-1 from Se-enriched Ganoderma lucidum

J Agric Food Chem. 2009 Sep 9;57(17):7737-42. doi: 10.1021/jf9019344.


Inorganic selenite can be transformed into organic forms and bind to proteins and polysaccharides in Se-enriched submerged Ganoderma lucidum cultures. In the present study, a novel Se-containing polysaccharide, SeGLP-2B-1, was purified from the Se-enriched mycelia of G. lucidum and the antiproliferative activities against six human cancer cell lines were investigated. The Se content of SeGLP-2B-1 was 186.7 microg/g, which was 150-fold larger than that of the regular polysaccharide GLP-2B-1 (1.3 microg/g). SeGLP-2B-1 (1.06 x 10(6) Da) was composed of glucose, rhamnose, xylose, and galactose with a molar ratio of 1.000:0.652:0.443:0.227. SeGLP-2B-1 exhibited an approximately 10-fold stronger antiproliferative activity against six human cancer cell lines as compared to GLP-2B-1. Thus, Se is believed to play an important role in increasing the antiproliferative property of SeGLP-2B-1. These findings indicate that SeGLP-2B-1 may serve as a dietary Se supplement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Breast Neoplasms
  • Carcinoma, Hepatocellular
  • Cell Cycle / drug effects
  • Cell Division / drug effects*
  • Cell Line, Tumor
  • Chemical Phenomena
  • Dietary Supplements
  • Female
  • Ganoderma / chemistry*
  • HeLa Cells
  • Humans
  • K562 Cells
  • Liver Neoplasms
  • Mycelium / chemistry*
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / isolation & purification*
  • Organometallic Compounds / pharmacology*
  • Polysaccharides / analysis
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification*
  • Polysaccharides / pharmacology*
  • Selenium / administration & dosage*
  • Selenium / analysis


  • Antineoplastic Agents
  • Organometallic Compounds
  • Polysaccharides
  • SeGLP-2B-1
  • Selenium