A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis

Andrea Ciammaruconi; Saverio Grassi; Giovanni Faggioni; Riccardo De Santis; Valentina Pittiglio; Raffaele D'Amelio; Gilles Vergnaud; Florigio Lista

doi:10.1016/j.diagmicrobio.2009.04.015

A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve analysis

Diagn Microbiol Infect Dis. 2009 Sep;65(1):7-13. doi: 10.1016/j.diagmicrobio.2009.04.015.

Authors

Andrea Ciammaruconi¹, Saverio Grassi, Giovanni Faggioni, Riccardo De Santis, Valentina Pittiglio, Raffaele D'Amelio, Gilles Vergnaud, Florigio Lista

Affiliation

¹ Army Medical Research Center, 00184 Rome, Italy. andrea.ciammaruconi@gmail.com

PMID: 19679229
DOI: 10.1016/j.diagmicrobio.2009.04.015

Abstract

Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing technique permits to differentiate Y. pestis isolates and turned out to be robust, reproducible, and cheap.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't
Review

MeSH terms

Alleles*
Bacterial Typing Techniques / methods*
DNA, Bacterial / genetics*
Genotype
Humans
Polymorphism, Genetic*
Transition Temperature*
Yersinia pestis / classification*
Yersinia pestis / genetics*

Substances

DNA, Bacterial