Superoxide protects Escherichia coli from bleomycin mediated lethality

J Inorg Biochem. 2009 Sep;103(9):1273-7. doi: 10.1016/j.jinorgbio.2009.07.009. Epub 2009 Jul 17.

Abstract

Superoxide and its products, especially hydroxyl radical, were recently proposed to be instrumental in cell death following treatment with a wide range of antimicrobials. Surprisingly, bleomycin lethality to Escherichia coli was ameliorated by a genetic deficiency of superoxide dismutase or by furnishing the superoxide generator plumbagin. Rescue by plumbagin was similar in strains containing or lacking recA or with inactive, inducible, or constitutive soxRS regulons. Thus, superoxide interferes with bleomycin cytotoxicity in ways not readily explained by genetic pathways expected to protect from oxidative damage.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bleomycin / administration & dosage
  • Bleomycin / pharmacology*
  • Escherichia coli / drug effects*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism
  • Naphthoquinones / metabolism
  • Naphthoquinones / pharmacology
  • Rec A Recombinases / genetics
  • Rec A Recombinases / metabolism
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • Superoxides / metabolism*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transduction, Genetic
  • Transformation, Bacterial

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Escherichia coli Proteins
  • Mutant Proteins
  • Naphthoquinones
  • Trans-Activators
  • Transcription Factors
  • Bleomycin
  • Superoxides
  • SoxR protein, Bacteria
  • SoxS protein, E coli
  • SodA protein, Bacteria
  • SodB protein, Bacteria
  • Superoxide Dismutase
  • Rec A Recombinases
  • plumbagin