HAHA--nothing to laugh about. Measuring the immunogenicity (human anti-human antibody response) induced by humanized monoclonal antibodies applying ELISA and SPR technology

J Pharm Biomed Anal. 2010 Jan 5;51(1):252-4. doi: 10.1016/j.jpba.2009.07.013. Epub 2009 Jul 22.


Immunogenicity induced by passively applied proteins is a serious issue because it is directly related to the patient's safety. The out-come of an immune reaction to a therapeutic protein can range from transient appearance of antibodies without any clinical significance to severe life threatening conditions. Within this article, enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) methodology to measure immunogenicity are compared and the pros and cons are discussed.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Immunotherapy / methods
  • Surface Plasmon Resonance / methods*


  • Antibodies, Monoclonal