A Novel Octamer Binding Transcription Factor Is Differentially Expressed in Mouse Embryonic Cells

Cell. 1990 Feb 9;60(3):461-72. doi: 10.1016/0092-8674(90)90597-8.

Abstract

We have identified a novel octamer binding factor (Oct-3) in P19 embryonal carcinoma cells. Oct-3, which recognizes the typical octamer motif (ATTTGCAT) as well as the AT-rich sequence TTAAAATTCA, is present in P19 stem cells but disappears when the cells are induced to differentiate by retinoic acid (RA). Cloned cDNA corresponding to Oct-3 encodes a protein of 377 amino acids. Oct-3 has a conserved POU domain, but the remaining part is distinct from other POU domain-containing proteins such as Oct-1 and Oct-2. mRNA of 1.5 kb coding for Oct-3 is abundant in P19 stem cells but is dramatically repressed during RA-induced differentiation. Repression of the 1.5 kb mRNA is rapid and specific to RA. In mouse, oct-3 mRNA is undetectable in all the adult organs examined. The N-terminal proline-rich region of Oct-3, when fused to the DNA binding domain of c-Jun, functions as a transcriptional activating domain. We suggest that Oct-3 is a novel octamer binding transcription factor that is developmentally regulated during mouse embryogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Enhancer Elements, Genetic*
  • Gene Library
  • Genes, Homeobox
  • Genetic Vectors
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Octamer Transcription Factor-3
  • Oligonucleotide Probes
  • Plasmids
  • Protein Biosynthesis
  • RNA, Neoplasm / genetics
  • Sequence Homology, Nucleic Acid
  • Teratoma
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Octamer Transcription Factor-3
  • Oligonucleotide Probes
  • RNA, Neoplasm
  • Transcription Factors

Associated data

  • GENBANK/J03178
  • GENBANK/M34381