Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor

Cell. 1990 Feb 23;60(4):535-45. doi: 10.1016/0092-8674(90)90657-z.


The gene (E2A) for enhancer binding transcription factors E12 and E47 maps to the t(1;19) chromosomal translocation breakpoint in pre-B cell leukemias. Altered E2A transcripts lacking sequences coding for the helix-loop-helix DNA binding motif were detected in several t(1;19)-carrying cell lines. Fusion cDNAs that crossed the t(1;19) breakpoint were cloned and shown to code for an 85 kd protein consisting of the amino-terminal two-thirds of E2A fused to a chromosome 1-derived protein. The fusion protein has the features of a chimeric transcription factor in which the DNA binding domain of E2A is replaced by the putative DNA binding domain of a homeoprotein from chromosome 1 for which the name Prl (pre-B cell leukemia) is proposed. Identical E2A-prl mRNA junctions were detected by PCR in three t(1;19)-carrying cell lines, indicating that the fusion transcripts and predicted chimeric protein are a consistent feature of this translocation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Burkitt Lymphoma / genetics
  • Cell Line
  • Chimera
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • Chromosomes, Human, Pair 19*
  • Cloning, Molecular
  • DNA Probes
  • DNA, Neoplasm / genetics
  • Genes, Homeobox*
  • Humans
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • RNA, Messenger / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription Factors / genetics*
  • Translocation, Genetic*


  • DNA Probes
  • DNA, Neoplasm
  • Oligonucleotide Probes
  • RNA, Messenger
  • Transcription Factors

Associated data

  • GENBANK/A33061
  • GENBANK/B33061
  • GENBANK/M31170
  • GENBANK/M31222