The miRNA-192/194 cluster regulates the Period gene family and the circadian clock

FEBS J. 2009 Oct;276(19):5447-55. doi: 10.1111/j.1742-4658.2009.07229.x. Epub 2009 Aug 13.


Several biological functions in mammals are regulated in a circadian fashion. The molecular mechanisms orchestrating these circadian rhythms have been unravelled. The biological clock, with its core transcriptional unit Bmal1/CLOCK, is composed of several self-sustaining feedback loops. In this study, we describe another mechanism impinging on the core components of the circadian clock. Using a forward genetic screen, we identified the miR-192/194 cluster as a potent inhibitor of the entire Period gene family. In accordance, the exogenous expression of miR-192/194 leads to an altered circadian rhythm. Thus, our results have uncovered a new mechanism for the control of the circadian clock at the post-transcriptional level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Base Sequence
  • Cell Line
  • Circadian Rhythm / genetics*
  • DNA Primers / genetics
  • Genes, Reporter
  • Green Fluorescent Proteins / genetics
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Mice
  • MicroRNAs / genetics*
  • Multigene Family*
  • Mutation
  • Period Circadian Proteins


  • 3' Untranslated Regions
  • DNA Primers
  • Intracellular Signaling Peptides and Proteins
  • MicroRNAs
  • PER1 protein, human
  • Per1 protein, mouse
  • Period Circadian Proteins
  • Green Fluorescent Proteins