Combination treatment with resveratrol and sulforaphane induces apoptosis in human U251 glioma cells

Neurochem Res. 2010 Jan;35(1):152-61. doi: 10.1007/s11064-009-0040-7. Epub 2009 Aug 15.

Abstract

Resveratrol is a naturally occurring polyphenolic compound highly enriched in grapes, peanuts, red wine, and a variety of food sources. Sulforaphane belongs to the family of isothiocyanates and is highly enriched in cruciferous vegetables. Our previous study showed that resveratrol, when used at high concentrations, inhibited cell proliferation, caused the cell cycle arrest and induced apoptotic cell death in glioma cells. In the current study, we tested the effect of combination treatment with resveratrol and sulforaphane, when both were used at low concentrations, on cell proliferation, migration and death in human U251 glioma cells. Our study shows that combination treatment with resveratrol and sulforaphane inhibits cell proliferation and migration, reduces cell viability, induces lactate dehydrogenase release, decreases pro-survival Akt phosphorylation and increases caspase-3 activation. The use of combination of bioactive food components, such as resveratrol and sulforaphane, may be a viable approach for the treatment of glioma.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Apoptosis / drug effects*
  • Cell Cycle / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Glioma / pathology*
  • Humans
  • Isothiocyanates
  • Resveratrol
  • Stilbenes / pharmacology*
  • Thiocyanates / pharmacology*

Substances

  • Isothiocyanates
  • Stilbenes
  • Thiocyanates
  • sulforaphane
  • Resveratrol