Cytological analysis of meiosis in fixed Drosophila ovaries

Methods Mol Biol. 2009;558:197-216. doi: 10.1007/978-1-60761-103-5_12.

Abstract

Methods are described to analyze two different parts of the Drosophila ovary, which correspond to early stages (pachytene) and late stages (metaphase I and beyond) of meiosis. In addition to taking into account morphology, the techniques differ by fixation conditions and the method to isolate the tissue. Most of these methods are whole mounts, which preserve the three-dimensional structure.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Cytogenetic Analysis / methods
  • Cytological Techniques / methods*
  • Drosophila / cytology*
  • Drosophila / genetics
  • Embryo, Nonmammalian / cytology
  • Female
  • Meiosis / genetics
  • Meiosis / physiology*
  • Models, Biological
  • Ovary / cytology*
  • Ovary / metabolism
  • Tissue Fixation / methods