Objective: To establish the technical systems of callus induction and the cell suspension culture of Changium smyrnioides.
Method: Effects of different plant growth regulators on leaf, petiole and root callus induction were studied by orthogonal design, and cell suspension cultures from different explants were carried out.
Result: Petiole was optimal to form callus, followed by the root and leaf. Four kinds of plant growth regulators showed a significant effect on the petiole and root callus induction. The optimal plant growth regulators for petiole callus induction was 0.5 mg x L(-1) NAA, 0.5 mg x L(-1) 2,4-D, 0.1 mg x L(-1) KT and 0.5 mg x L(-1) 6-BA; and induction rate reached to 100% when petiole was cultured for 14 days. In addition, when cell from petiole callus was cultured for 21 days, the maximum yields of cell biomass and polysaccharide were 1090.28 mg x L(-1) and 36.85 mg x L(-1), respectively.
Conclusion: Petiole was taken as the optimal explant for inducing callus and the optimal cell origin for the cell suspension culture of C. smyrnioides.