G-quadruplex DNA bound by a synthetic ligand is highly dynamic

J Am Chem Soc. 2009 Sep 9;131(35):12522-3. doi: 10.1021/ja903408r.


Using single molecule fluorescence resonance energy transfer, we investigated the interaction between a quadruplex-binding ligand and the human telomeric G-quadruplex. The binding of quinolinecarboxamide macrocycle to telomeric DNA was essentially irreversible and selectively induced and favored one quadruplex conformation. The ligand-quadruplex complex displayed intramolecular dynamics including quadruplex folding and unfolding in the absence of ligand association and dissociation. We report that the G-quadruplex can be stabilized without preventing the intrinsic intramolecular dynamics of telomeric DNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Avidin / metabolism
  • Base Sequence
  • Biotin / metabolism
  • Cattle
  • DNA / chemistry*
  • DNA / genetics
  • DNA / metabolism*
  • Fluorescence Resonance Energy Transfer
  • G-Quadruplexes*
  • Humans
  • Ligands
  • Quartz / chemistry
  • Telomere / genetics


  • Ligands
  • neutravidin
  • Avidin
  • Quartz
  • Biotin
  • DNA