An in vitro predictive test for clinical graft-versus-host disease in allogeneic bone marrow transplant recipients

Bone Marrow Transplant. 1990 Feb;5(2):105-9.


An in vitro skin explant model has been used in an attempt to predict the severity of graft-versus-host disease (GVHD) in HLA-identical donor-recipient pairs. The skin explant model involves the use of donor lymphocytes which have been sensitized against recipient lymphocytes in vitro and then co-cultured with the recipient's skin. Thirteen patients were studied in a prospective manner and results from the skin explant model compared with the clinical status of the patient post-transplant showed good correlation (p less than 0.001). Results from T cell-depletion studies indicated a role for both CD4 and CD8 positive cells in GVHD. In conclusion the results confirmed that the skin explant assay is a useful and predictive test of GVHD in humans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biological Assay / methods
  • Bone Marrow Transplantation* / adverse effects
  • CD4-Positive T-Lymphocytes / immunology
  • Culture Techniques
  • Graft vs Host Disease / diagnosis*
  • Graft vs Host Disease / etiology
  • Humans
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / surgery*
  • Leukemia, Myeloid, Acute / surgery*
  • Lymphocyte Culture Test, Mixed
  • Lymphocyte Depletion
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / surgery*
  • Predictive Value of Tests
  • Prospective Studies
  • Skin / immunology*
  • T-Lymphocytes, Regulatory / immunology