Genome scale reconstruction of a Salmonella metabolic model: comparison of similarity and differences with a commensal Escherichia coli strain

J Biol Chem. 2009 Oct 23;284(43):29480-8. doi: 10.1074/jbc.M109.005868. Epub 2009 Aug 18.


Salmonella are closely related to commensal Escherichia coli but have gained virulence factors enabling them to behave as enteric pathogens. Less well studied are the similarities and differences that exist between the metabolic properties of these organisms that may contribute toward niche adaptation of Salmonella pathogens. To address this, we have constructed a genome scale Salmonella metabolic model (iMA945). The model comprises 945 open reading frames or genes, 1964 reactions, and 1036 metabolites. There was significant overlap with genes present in E. coli MG1655 model iAF1260. In silico growth predictions were simulated using the model on different carbon, nitrogen, phosphorous, and sulfur sources. These were compared with substrate utilization data gathered from high throughput phenotyping microarrays revealing good agreement. Of the compounds tested, the majority were utilizable by both Salmonella and E. coli. Nevertheless a number of differences were identified both between Salmonella and E. coli and also within the Salmonella strains included. These differences provide valuable insight into differences between a commensal and a closely related pathogen and within different pathogenic strains opening new avenues for future explorations.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Genome, Bacterial / physiology
  • Models, Biological*
  • Oligonucleotide Array Sequence Analysis
  • Open Reading Frames / physiology
  • Salmonella enteritidis / genetics*
  • Salmonella enteritidis / metabolism*
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / metabolism*
  • Species Specificity