Type I pro-collagen promoting and anti-collagenase activities of Phyllanthus emblica extract in mouse fibroblasts

J Cosmet Sci. 2009 Jul-Aug;60(4):395-403.


As part of an ongoing search for the novel pharmacological activities of Phyllanthus emblica, the present study has shown its type I collagen promoting and anti-collagenase effects on primary mouse fibroblast cells. At a concentration of 0.1 mg/ml, emblica extract significantly increased the type I pro-collagen level up to 1.65-fold, and 6.78-fold greater than that of an untreated control, determined by immunocytochemistry and Western blot analysis, respectively. Emblica extract caused an approximately 7.75-fold greater type I pro-collagen induction compared to the known herbal collagen enhancer asiaticoside at the same treatment concentration (0.1 mg/ml). Moreover, emblica extract inhibited collagenase activity in a dose-dependent manner. Maximal inhibition was observed (78.67 +/- 3.51%) at a concentration of 1 mg/ml. In summary, emblica extract has a promising pharmacological effect that benefits collagen synthesis and protects against its degradation and could be used as a natural anti-aging ingredient.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Survival / drug effects
  • Collagen Type I / biosynthesis*
  • Collagenases / metabolism
  • Female
  • Fibroblasts / drug effects*
  • Fibroblasts / enzymology
  • Fibroblasts / metabolism
  • Fruit / metabolism
  • Immunohistochemistry
  • Matrix Metalloproteinase Inhibitors*
  • Mice
  • Phyllanthus emblica / chemistry*
  • Phyllanthus emblica / metabolism
  • Plant Extracts / pharmacology*
  • Skin Aging / drug effects


  • Collagen Type I
  • Matrix Metalloproteinase Inhibitors
  • Plant Extracts
  • Collagenases