Human carboxypeptidase A identifies a BglII RFLP and maps to 7q31-qter

Am J Hum Genet. 1990 Apr;46(4):795-800.


A genomic clone for human carboxypeptidase has been isolated with a probe for rat CPA1 cDNA. A 1.7-kb HindIII/EcoRI fragment from the 3' flanking region of human carboxypeptidase detects a DNA polymorphism with BglIII. Multipoint linkage analysis with an established map of chromosome 7 markers shows that the most likely location of carboxypeptidase is at 7q31-qter, between D7S87 and D7S93. All other placements can be excluded with odds greater than 100:1. These and other markers confirm that carboxypeptidase lies distal to the locus for cystic fibrosis, at a distance of approximately 12 centimorgans. The regions containing identity to the rat gene were sequenced and shown to be 82% identical to exons 9 and 10 of the rat gene. The presence of a codon for isoleucine at the residues corresponding to codon 255 of rat CPA1 cDNA strongly suggests that the A form of human carboxypeptidase has been isolated.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • Base Sequence
  • Carboxypeptidases / genetics*
  • Carboxypeptidases A
  • Chromosomes, Human, Pair 7*
  • DNA / genetics
  • Deoxyribonucleases, Type II Site-Specific*
  • Female
  • Genetic Markers
  • Humans
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Polymorphism, Restriction Fragment Length
  • Restriction Mapping


  • Bacterial Proteins
  • Genetic Markers
  • DNA
  • BglII endonuclease
  • Deoxyribonucleases, Type II Site-Specific
  • Carboxypeptidases
  • Carboxypeptidases A