Correlation of human leukocyte antigen-G (HLA-G) expression and disease progression in epithelial ovarian cancer

Reprod Sci. 2009 Nov;16(11):1103-11. doi: 10.1177/1933719109342131. Epub 2009 Aug 19.


Human leukocyte antigen-G (HLA-G) expression has been reported to be relevant to cancer development and immune tolerance. The purpose of this study was to investigate the correlation between HLA-G expression and disease progression and to assess the use of HLA-G expression as a prognostic immunomarker in epithelial ovarian carcinoma. Human leukocyte antigen-G expression in 41 ovarian cancer tissues and 8 normal ovarian tissues was analyzed using immunohistochemistry and Western blot assay. Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) was used for HLA-G messenger RNA (mRNA) expression. Human leukocyte antigen-G mRNA and protein levels were significantly greater in advanced ovarian cancer tissues than in normal or early stage ovarian cancer tissues (P < .05 and P < .05, respectively). Patients with HLA-G expression had a significantly worse prognosis. There is a significant correlation between HLA-G immunoreactivity and patient survival in univariate analysis (P = .04). Our data was consistent with the concept that HLA-G expression might play a pivotal role in the development and disease progression of epithelial ovarian cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Biomarkers, Tumor / metabolism
  • Blotting, Western
  • Carcinoma / metabolism*
  • Carcinoma / pathology
  • Disease Progression
  • Female
  • Flow Cytometry
  • HLA Antigens / metabolism*
  • HLA-G Antigens
  • Histocompatibility Antigens Class I / metabolism*
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Neoplasm Staging
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology
  • Ovary / metabolism*
  • Ovary / pathology
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Statistics, Nonparametric


  • Biomarkers, Tumor
  • HLA Antigens
  • HLA-G Antigens
  • Histocompatibility Antigens Class I
  • RNA, Messenger