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. 2009 Sep 25;325(5948):1693-6.
doi: 10.1126/science.1173759. Epub 2009 Aug 20.

Creating Bacterial Strains From Genomes That Have Been Cloned and Engineered in Yeast

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Creating Bacterial Strains From Genomes That Have Been Cloned and Engineered in Yeast

Carole Lartigue et al. Science. .
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Abstract

We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.

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