Cloning, characterization, and antifungal activity of an endo-1,3-beta-D: -glucanase from Streptomyces sp. S27

Appl Microbiol Biotechnol. 2010 Feb;85(5):1483-90. doi: 10.1007/s00253-009-2187-1. Epub 2009 Aug 21.

Abstract

An endo-1,3-beta-D: -glucanase gene, designated as bglS27, was cloned from Streptomyces sp. S27 and successfully expressed in Escherichia coli BL21 (DE3). The full-length gene contains 1,362 bp and encodes a protein of 453 amino acids with a calculated molecular mass of 42.7 kDa. The encoded protein comprises a catalytic module of glycosyl hydrolase family 16, a short glycine linker region, and a family 13 carbohydrate-binding module. The purified recombinant enzyme (BglS27) showed optimal activity at 65 degrees C and pH 5.5 and preferentially catalyzed the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action. The specific activity and K(m) value of BglS27 for laminarin were 236.0 U mg(-1) and 1.89 mg ml(-1), respectively. In antifungal assay, BglS27 had the ability to inhibit the growth of phytopathogenic fungi Rhizoctonic solani and Fusarium oxysporum and some mycotoxin-producing fungi Fusarium crookwellense and Paecilomyces variotii. These favorable properties make BglS27 a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacterial Proteins / pharmacology
  • Base Sequence
  • Cloning, Molecular*
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Fungi / drug effects*
  • Genes, Bacterial
  • Glucan Endo-1,3-beta-D-Glucosidase / chemistry
  • Glucan Endo-1,3-beta-D-Glucosidase / genetics
  • Glucan Endo-1,3-beta-D-Glucosidase / metabolism*
  • Glucan Endo-1,3-beta-D-Glucosidase / pharmacology*
  • Glucans / metabolism
  • Hydrolysis
  • Molecular Sequence Data
  • Polysaccharides / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Sequence Alignment
  • Streptomyces / enzymology*
  • Streptomyces / genetics
  • Streptomyces / metabolism
  • Substrate Specificity

Substances

  • Antifungal Agents
  • Bacterial Proteins
  • DNA, Bacterial
  • Glucans
  • Polysaccharides
  • Recombinant Proteins
  • laminaran
  • Glucan Endo-1,3-beta-D-Glucosidase

Associated data

  • GENBANK/FJ887899