Determination of mangiferin in rat plasma by liquid-liquid extraction with UPLC-MS/MS

J Pharm Biomed Anal. 2010 Jan 5;51(1):260-3. doi: 10.1016/j.jpba.2009.07.021. Epub 2009 Jul 24.


An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method employing electrospray ionization (ESI) has been developed for the determination of mangiferin in rat plasma using diphenhydramine as the internal standard (IS). Liquid-liquid extraction (LLE) was used for sample preparation and the analysis was achieved with gradient elution on C(18) reversed phase column. The method was validated over the concentration range 0.02-5.0 microg/mL for oral administration and 0.4-100 microg/mL for intravenous administration. The intra-day and inter-day precision of mangiferin expressed as RSD<15% and the accuracy (RE) did not exceed 15%. This validated method is a novel technique for sample preparation and quantitation, which was successfully applied to estimate the bioavailability of mangiferin.

Publication types

  • Validation Study

MeSH terms

  • Administration, Oral
  • Animals
  • Biological Availability
  • Chromatography, High Pressure Liquid / methods*
  • Diphenhydramine / chemistry
  • Dose-Response Relationship, Drug
  • Injections, Intravenous
  • Male
  • Rats
  • Rats, Wistar
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Tandem Mass Spectrometry / methods*
  • Xanthones / administration & dosage
  • Xanthones / blood*
  • Xanthones / pharmacokinetics


  • Xanthones
  • mangiferin
  • Diphenhydramine