Arrested maturation of excitatory synapses in autosomal dominant lateral temporal lobe epilepsy

Nat Med. 2009 Oct;15(10):1208-14. doi: 10.1038/nm.2019. Epub 2009 Aug 23.

Abstract

A subset of central glutamatergic synapses are coordinately pruned and matured by unresolved mechanisms during postnatal development. We report that the human epilepsy gene LGI1, encoding leucine-rich, glioma-inactivated protein-1 and mutated in autosomal dominant lateral temporal lobe epilepsy (ADLTE), mediates this process in hippocampus. We created transgenic mice either expressing a truncated mutant LGI1 (835delC) found in ADLTE or overexpressing a wild-type LGI1. We discovered that the normal postnatal maturation of presynaptic and postsynaptic functions was arrested by the 835delC mutant LGI1, and contrastingly, was magnified by excess wild-type LGI1. Concurrently, mutant LGI1 inhibited dendritic pruning and increased the spine density to markedly increase excitatory synaptic transmission. Inhibitory transmission, by contrast, was unaffected. Furthermore, mutant LGI1 promoted epileptiform discharge in vitro and kindling epileptogenesis in vivo with partial gamma-aminobutyric acid(A) (GABA(A)) receptor blockade. Thus, LGI1 represents a human gene mutated to promote epilepsy through impaired postnatal development of glutamatergic circuits.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Epilepsy, Temporal Lobe / genetics*
  • Gene Expression Regulation, Developmental
  • Genes, Dominant
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Mice, Transgenic
  • Mutation
  • Proteins / genetics*
  • Receptors, GABA-B / genetics
  • Synapses / genetics*
  • Transgenes

Substances

  • Intracellular Signaling Peptides and Proteins
  • LGI1 protein, human
  • Lgi1 protein, mouse
  • Proteins
  • Receptors, GABA-B