Simultaneous comparison of bilberry (Vaccinium myrtillus L.) and blueberry (Vaccinium corymbosum L) fruits for their anthocyanin composition, antimicrobial and antioxidant activity is reported. The aim of this study was to investigate and to compare anthocyanin composition, antimicrobial and antioxidant activity in bilberry and blueberry fruits and their skins. The investigations revealed that the highest amount of total anthocyanins was observed in fruits skins of blueberry cultivars. The results, obtained by chromatographic analysis, indicated that cyanidin is a dominant anthocyanidin in bilberry and malvidin in blueberry samples. Extracts of "Herbert", "Coville", "Toro" blueberry cultivars and bilberry fruits revealed antimicrobial properties. Citrobacter freundii (ATCC 8090) and Enterococcus faecalis (ATCC29212) were the most sensitive among eight tested Gram-negative and Gram-positive bacteria. Significant differences between berry and skin extracts were not established. Studies with fruits showed that the strongest antioxidant activity possesses blueberry cultivar "Berkeley" (82.13 +/- 0.51%). Meanwhile, the amount of quenched free radicals in bilberry samples was 63.72 +/- 1.11%, respectively. The lowest antioxidant activity was estimated in blueberry cultivar "Coville". Accordingly, the strongest antiradical properties were estimated in blueberry cultivar "Ama" fruit skins. Bilberry fruit skin samples possess strong antiradical activity as well (82.69 +/- 0.37%).