Flavonoids of Herba Epimedii Regulate Osteogenesis of Human Mesenchymal Stem Cells Through BMP and Wnt/beta-catenin Signaling Pathway

Mol Cell Endocrinol. 2010 Jan 15;314(1):70-4. doi: 10.1016/j.mce.2009.08.012. Epub 2009 Aug 22.

Abstract

Herba Epimedii is one of the most commonly used Chinese herbs for treating osteoporosis. In the present study, the flavonoids of Herba Epimedii (HEF) have shown to promote the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. They were noted to enhance the mRNA expression of BMP-2, BMP-4, Runx2, beta-catenin and cyclinD1, all of which are BMP or Wnt-signaling pathway related regulators. The osteogenic effect was inhibited by the introduction of noggin and DKK-1, which is classical inhibitor of BMP and Wnt/beta-catenin signaling, respectively. These results suggest that HEF exerts promoting effect on osteogenic differentiation, which plausibly functions via the BMP and Wnt/beta-catenin signaling pathways. Considering the therapeutic efficiency and economical issues, HEF may be a potential candidate for promoting bone regeneration. On the other hand, osteogenic differentiation of MSCs may also be a promising and attractive tool to apply in bone repair.

MeSH terms

  • Alkaline Phosphatase / genetics
  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone Morphogenetic Protein 2 / genetics
  • Bone Morphogenetic Protein 2 / metabolism
  • Bone Morphogenetic Protein 4 / genetics
  • Bone Morphogenetic Protein 4 / metabolism
  • Bone Morphogenetic Proteins / metabolism*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology
  • Cell Proliferation / drug effects
  • Core Binding Factor Alpha 1 Subunit / genetics
  • Core Binding Factor Alpha 1 Subunit / metabolism
  • Cyclin D1 / genetics
  • Cyclin D1 / metabolism
  • Drugs, Chinese Herbal / chemistry
  • Epimedium / chemistry*
  • Female
  • Flavonoids / pharmacology*
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Medicine, Chinese Traditional
  • Mesenchymal Stem Cells* / cytology
  • Mesenchymal Stem Cells* / drug effects
  • Mesenchymal Stem Cells* / physiology
  • Osteogenesis* / drug effects
  • Osteogenesis* / physiology
  • Signal Transduction* / drug effects
  • Signal Transduction* / physiology
  • Wnt Proteins / genetics
  • Wnt Proteins / metabolism*
  • beta Catenin / genetics
  • beta Catenin / metabolism*

Substances

  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins
  • Carrier Proteins
  • Core Binding Factor Alpha 1 Subunit
  • DKK1 protein, human
  • Drugs, Chinese Herbal
  • Flavonoids
  • Intercellular Signaling Peptides and Proteins
  • Wnt Proteins
  • beta Catenin
  • Cyclin D1
  • noggin protein
  • Alkaline Phosphatase