Association of CD2 and CD45 on human T lymphocytes

Nature. 1990 May 3;345(6270):71-4. doi: 10.1038/345071a0.


At least two membrane receptors have been defined through which human T lymphocytes can be induced to proliferate and differentiate, namely the CD3-Ti antigen receptor complex and the CD2 molecule. Monoclonal antibodies directed at either CD2 or CD3 induce intracellular second messenger production and subsequent protein phosphorylation. On most human non-B lymphocytes, CD3-Ti and CD2 are coexpressed and seem to be functionally interrelated. But there are minor subpopulations in which these receptor systems can transduce signals despite a mutually exclusive expression, indicating that CD3-Ti and CD2 can act independently of each other. This view is supported by the finding that most monoclonal antibodies directed at the CD45 molecules are strongly co-mitogenic with CD2 but not CD3 monoclonal antibodies. As the intracytoplasmic domains of CD45 have tyrosine phosphatase activity these functional effects could be explained by a physical association between CD2 and CD45. Using chemical crosslinking techniques, we now show that CD45 is linked to CD2 on the surface of human T lymphocytes.

MeSH terms

  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / immunology*
  • Antigens, Differentiation / immunology*
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • CD2 Antigens
  • CD3 Complex
  • Cross-Linking Reagents / pharmacology
  • Enzyme-Linked Immunosorbent Assay
  • Histocompatibility Antigens / immunology*
  • Histocompatibility Antigens Class I / immunology
  • Humans
  • Immunosorbent Techniques
  • Leukocyte Common Antigens
  • Molecular Weight
  • Peptide Fragments
  • Phosphoprotein Phosphatases / metabolism
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Protein Tyrosine Phosphatases
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Immunologic / immunology*
  • Second Messenger Systems
  • Serine Endopeptidases
  • Succinimides / pharmacology
  • T-Lymphocytes / immunology*


  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation
  • Antigens, Differentiation, T-Lymphocyte
  • CD2 Antigens
  • CD3 Complex
  • Cross-Linking Reagents
  • Histocompatibility Antigens
  • Histocompatibility Antigens Class I
  • Peptide Fragments
  • Phosphoproteins
  • Receptors, Antigen, T-Cell
  • Receptors, Immunologic
  • Succinimides
  • Phosphoprotein Phosphatases
  • Leukocyte Common Antigens
  • Protein Tyrosine Phosphatases
  • Serine Endopeptidases
  • glutamyl endopeptidase
  • dithiobis(succinimidylpropionate)