Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription

Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14265-70. doi: 10.1073/pnas.0900162106. Epub 2009 Aug 11.

Abstract

Human PC4 and the yeast ortholog Sub1 have multiple functions in RNA polymerase II transcription. Genome-wide mapping revealed that Sub1 is present on Pol III-transcribed genes. Sub1 was found to interact with components of the Pol III transcription system and to stimulate the initiation and reinitiation steps in a system reconstituted with all recombinant factors. Sub1 was required for optimal Pol III gene transcription in exponentially growing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Far-Western
  • Chromatin Immunoprecipitation
  • Chromosome Mapping
  • Chromosomes, Fungal / genetics
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Fungal
  • Genome, Fungal
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Protein Binding
  • RNA Polymerase III / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription Factors, TFIII / metabolism
  • Transcription, Genetic*

Substances

  • DNA, Fungal
  • DNA-Binding Proteins
  • SUB1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Transcription Factors, TFIII
  • transcription factor TFIIIC
  • RNA Polymerase III