Milk from buffalo, cow, goat and camel species was collected in Ismailia in Egypt. Aflatoxin (AFM(1)) levels were lower than previous surveys, and were influenced by feeding practices. Cows and buffaloes are fed prepared rations and had highest incidence of AFM(1). Camels forage freely on available pasture and had lowest AFM(1) in their milk. Goats are fed a combination of prepared ration as a supplement to pasture grazing. Most milks (80%, 74%, 66% and 52% of the camel, goat, cow and buffalo milks, respectively) were below the European Union maximum of AFM(1) <50 ng/L and all milk samples were <500 ng/L.