Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae

Infect Immun. 1990 Jun;58(6):1995-8. doi: 10.1128/iai.58.6.1995-1998.1990.


Type 1 fimbriae of Escherichia coli are surface organelles which mediate binding to D-mannose-containing structures. By direct binding of FimH to D-mannose attached to a carrier protein, we demonstrated that this protein was uniquely responsible for the receptor specificity. Furthermore, we show by receptor immunoelectron microscopy that the FimH protein is located laterally in the structure of the type 1 fimbriae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Escherichia coli
  • Bacterial Adhesion*
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Outer Membrane Proteins / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Fimbriae Proteins*
  • Fimbriae, Bacterial / metabolism*
  • Genes, Bacterial
  • Mannose / metabolism*
  • Microscopy, Electron
  • Molecular Weight
  • Plasmids
  • Recombinant Proteins


  • Adhesins, Escherichia coli
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Recombinant Proteins
  • fimH protein, E coli
  • Fimbriae Proteins
  • Mannose