LC determination of albiflorin and paeoniflorin in Bai Shao (Paeonia lactiflora) as a raw herb and dried aqueous extract

J AOAC Int. Jul-Aug 2009;92(4):1027-34.


A validated analytical method is reported for the analysis of paeoniflorin and albiflorin in Bai Shao (Paeonia lactiflora) as a dried raw herb and commercially prepared dried aqueous extract. The samples were extracted by sonication in methanol and the extract analyzed by LC-photodiode array with identity confirmation by electrospray ionization-tandem MS. A C18 column was used with an acetonitrile-water gradient mobile phase. Paeoniflorin and albiflorin were quantified at 230 nm. Ions with m/z 121 and 327 were produced with the MS detector, using the paeoniflorin precursor ion with m/z 479. For albiflorin, the precursor ion with m/z 479 produced the m/z 121 and 77 ions. The amounts of paeoniflorin and albiflorin found in the raw herb were 33.2 and 1.8 mg/g, respectively; and in the dried aqueous extract, the amounts were 34.8 and 15.7 mg/g, respectively. The LODs for paeoniflorin and albiflorin were 0.37 and 1.39 mg/g, respectively, for the raw herb and 0.25 and 0.06 mg/g, respectively, for the dried aqueous extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzoates / analysis*
  • Bridged-Ring Compounds / analysis*
  • Chromatography, Liquid
  • Chromatography, Thin Layer
  • Glucosides / analysis*
  • Indicators and Reagents
  • Medicine, Chinese Traditional
  • Monoterpenes
  • Paeonia / chemistry*
  • Plant Extracts / analysis
  • Plant Preparations / analysis
  • Reference Standards
  • Solvents
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrophotometry, Ultraviolet


  • Benzoates
  • Bridged-Ring Compounds
  • Glucosides
  • Indicators and Reagents
  • Monoterpenes
  • Plant Extracts
  • Plant Preparations
  • Solvents
  • peoniflorin
  • albiflorin