Applications of new sequencing technologies for transcriptome analysis

Annu Rev Genomics Hum Genet. 2009;10:135-51. doi: 10.1146/annurev-genom-082908-145957.


Transcriptome analysis has been a key area of biological inquiry for decades. Over the years, research in the field has progressed from candidate gene-based detection of RNAs using Northern blotting to high-throughput expression profiling driven by the advent of microarrays. Next-generation sequencing technologies have revolutionized transcriptomics by providing opportunities for multidimensional examinations of cellular transcriptomes in which high-throughput expression data are obtained at a single-base resolution.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • DNA / analysis
  • Gene Expression Profiling / instrumentation
  • Gene Expression Profiling / methods*
  • Genome
  • Humans
  • Polymorphism, Single Nucleotide
  • RNA, Untranslated / analysis
  • Sequence Analysis, DNA / instrumentation
  • Sequence Analysis, DNA / methods*


  • RNA, Untranslated
  • DNA