HIV inactivation by cross-linking of photo-labeled anti-retroviral compounds with HIV reverse transcriptase

Vaccine. 2009 Oct 19;27(44):6137-42. doi: 10.1016/j.vaccine.2009.08.028. Epub 2009 Aug 26.


We describe a new method for the development of a preventive inactivated-HIV vaccine, based on photo-inactivation of HIV reverse transcriptase (RT), which preserves both the conformational and functional integrity of viral surface proteins. The RT of HIV-1 was selectively targeted for inactivation using a photo-labeled compound with specific affinity for HIV-1 RT. The photo-labeled virions were then exposed to UV light causing the photo-labeled compound to form a covalent bond cross-linking the photo-active compound to RT. Replication capacity of the treated virions was significantly reduced when compared to controls suggesting that exposure of treated virions to UV light had caused a stable interaction of RT and the photo-labeling compound.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / chemistry*
  • Cell Line, Tumor
  • Cell Survival
  • Cross-Linking Reagents
  • HIV Core Protein p24 / analysis
  • HIV Infections
  • HIV Reverse Transcriptase / radiation effects*
  • HIV-1 / radiation effects*
  • Humans
  • Inhibitory Concentration 50
  • Photoaffinity Labels
  • Reverse Transcriptase Inhibitors / chemistry*
  • Substrate Specificity
  • Ultraviolet Rays
  • Virus Inactivation*


  • AIDS Vaccines
  • Cross-Linking Reagents
  • HIV Core Protein p24
  • Photoaffinity Labels
  • Reverse Transcriptase Inhibitors
  • p24 protein, Human Immunodeficiency Virus Type 1
  • reverse transcriptase, Human immunodeficiency virus 1
  • HIV Reverse Transcriptase