Gastrin secretion from human antral G cells in culture

Gastroenterology. 1990 Jul;99(1):36-44. doi: 10.1016/0016-5085(90)91226-v.


Receptor-dependent and -independent regulation of gastrin secretion from cultured human antral G cells was investigated. Human antral mucosal cell preparations that were enriched for G cells were obtained by sequential incubations with collagenase and ethylenediaminetetraacetic acid, centrifugal elutriation, and short-term culture. After a 2-day incubation period, gastrin- and somatostatin-containing cells accounted for 15% and 5%, respectively, of the total adhered-cell population. Forskolin, A23187, and beta-phorbol 12 myristate 13-acetate stimulated basal gastrin secretion from cultured human G cells in a concentration-dependent fashion. These results indicate that gastrin release could be mediated by elevations in cytosolic cyclic adenosine monophosphate levels, calcium influx, or activation of protein kinase C. A direct stimulatory role for bombesin- and gastrin-releasing peptide was supported by experiments showing concentration-dependent enhancement of gastrin release by bombesin from 0.01 fmol/L to 10 nmol/L. The putative bombesin antagonist [Leu13-psi-CH2NH-Leu14] bombesin augmented basal gastrin levels by itself and produced weak inhibition of bombesin-induced gastrin secretion from human antral G cells. Somatostatin potently suppressed forskolin- and bombesin-mediated gastrin release but did not significantly alter basal gastrin levels. These results suggest that bombesin and somatostatin directly activate and inhibit G-cell function via specific and sensitive receptors. Furthermore, the adenylate cyclase and phosphatidyl inositide second messenger systems seem to be intracellular mediators of gastrin secretion from human antral G cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bombesin / pharmacology
  • Calcimycin / pharmacology
  • Cells, Cultured
  • Chromaffin System / metabolism*
  • Colforsin / pharmacology
  • Enterochromaffin Cells / drug effects
  • Enterochromaffin Cells / metabolism*
  • Gastrins / metabolism*
  • Humans
  • Pyloric Antrum / metabolism
  • Receptors, Gastrointestinal Hormone / drug effects
  • Somatostatin / pharmacology
  • Stimulation, Chemical
  • Tetradecanoylphorbol Acetate / pharmacology


  • Gastrins
  • Receptors, Gastrointestinal Hormone
  • Colforsin
  • Calcimycin
  • Somatostatin
  • Tetradecanoylphorbol Acetate
  • Bombesin