Homology modelling and spectroscopy, a never-ending love story

Eur Biophys J. 2010 Mar;39(4):551-63. doi: 10.1007/s00249-009-0531-0. Epub 2009 Aug 29.


Homology modelling is normally the technique of choice when experimental structure data are not available but three-dimensional coordinates are needed, for example, to aid with detailed interpretation of results of spectroscopic studies. Herein, the state of the art of homology modelling will be described in the light of a series of recent developments, and an overview will be given of the problems and opportunities encountered in this field. The major topic, the accuracy and precision of homology models, will be discussed extensively due to its influence on the reliability of conclusions drawn from the combination of homology models and spectroscopic data. Three real-world examples will illustrate how both homology modelling and spectroscopy can be beneficial for (bio)medical research.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Humans
  • Models, Molecular*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Proteins / chemistry
  • Proteins / metabolism
  • Sequence Homology*
  • Spectrum Analysis / methods*
  • Spin Labels


  • Proteins
  • Spin Labels