Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin

Ann-Kathrin Marguerre; Roland Krämer

doi:10.1016/j.bmcl.2009.07.152

Lanthanide-based fluorogenic peptide substrate for the highly sensitive detection of thermolysin

Bioorg Med Chem Lett. 2009 Oct 1;19(19):5757-9. doi: 10.1016/j.bmcl.2009.07.152. Epub 2009 Aug 4.

Authors

Ann-Kathrin Marguerre¹, Roland Krämer

Affiliation

¹ Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.

PMID: 19720529
DOI: 10.1016/j.bmcl.2009.07.152

Abstract

A new fluorogenic, lanthanide-based oligopeptide substrate for the detection of the zinc-dependent endoprotease thermolysin is described. Using time-resolved fluorescence measurement, a highly sensitive assay for thermolysin was developed with a 50pM detection limit (3.5fmol).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Europium / chemistry*
Fluorescent Dyes / chemistry*
Peptides / chemical synthesis
Peptides / chemistry*
Spectrometry, Fluorescence / methods*
Thermolysin / analysis*
Zinc / chemistry

Substances

Fluorescent Dyes
Peptides
Europium
Thermolysin
Zinc