Paraspeckles: nuclear bodies built on long noncoding RNA

J Cell Biol. 2009 Sep 7;186(5):637-44. doi: 10.1083/jcb.200906113. Epub 2009 Aug 31.

Abstract

Paraspeckles are ribonucleoprotein bodies found in the interchromatin space of mammalian cell nuclei. These structures play a role in regulating the expression of certain genes in differentiated cells by nuclear retention of RNA. The core paraspeckle proteins (PSF/SFPQ, P54NRB/NONO, and PSPC1 [paraspeckle protein 1]) are members of the DBHS (Drosophila melanogaster behavior, human splicing) family. These proteins, together with the long nonprotein-coding RNA NEAT1 (MEN-epsilon/beta), associate to form paraspeckles and maintain their integrity. Given the large numbers of long noncoding transcripts currently being discovered through whole transcriptome analysis, paraspeckles may be a paradigm for a class of subnuclear bodies formed around long noncoding RNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • DNA-Binding Proteins
  • Gene Expression Regulation
  • Humans
  • Intranuclear Inclusion Bodies / genetics*
  • Intranuclear Inclusion Bodies / metabolism
  • Nuclear Matrix-Associated Proteins / genetics
  • Nuclear Matrix-Associated Proteins / metabolism
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Octamer Transcription Factors / genetics
  • Octamer Transcription Factors / metabolism
  • PTB-Associated Splicing Factor
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism

Substances

  • DNA-Binding Proteins
  • NONO protein, human
  • Nuclear Matrix-Associated Proteins
  • Nuclear Proteins
  • Octamer Transcription Factors
  • PSPC1 protein, human
  • PTB-Associated Splicing Factor
  • RNA, Untranslated
  • RNA-Binding Proteins