Preventive effect of low molecular weight glycosaminoglycan from Amussium pleuronectus (Linne) on oxidative injury and cellular abnormalities in isoproterenol-induced cardiotoxicity in Wistar rats

Appl Biochem Biotechnol. 2010 Sep;162(1):43-51. doi: 10.1007/s12010-009-8750-5. Epub 2009 Sep 2.


The present work explores suspicious consequence of low molecular weight glycosaminoglycan (LMW-GAG) on oxidative stress and cellular abnormalities in isoproterenol (ISO)-induced myocardial infarction in an experimental model. Group-III male Wistar rats (140 +/- 10 g) were administrated by ISO (85 mg ISO/ml subcutaneously (SC) injected at the last two days of a 2-week period). Group-IV rats were treated LMW-GAG plus ISO (300 microg/day per rat SC for 1 week followed by 85 mg/kg ISO on the end last two days of the 2 - weeks). Untreated control (Group-I) and LMW-GAG drug control (Group-II) were also included. Serum and tissue lactate dehydrogenase, aminotransferases, and creatine kinase activities were increased in ISO group, which were normalized by LMW-GAG pretreatment rats. Antioxidant enzymes - superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities and non-enzymatic enzyme reduced glutathione (GSH) were decreased in the ISO induced rats, and this was increased by LMW-GAG pretreatment. Increased level of thiobarbituric acid reactive substances (TBARS) in plasma and the heart of ISO treated rats; pre s.c. injected with LMW-GAG to ISO-induced rats decreased the levels of TBARS. Histological examination revealed that the ISO-induced deleterious changes in the heart tissues were offset by LMW-GAG treatment. LMW-GAG affords considerable protection to the tissues challenged by cardiotoxicity, evidenced by its correction and restoration of serum and tissue indices of injury, to normalcy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Glycosaminoglycans / chemistry*
  • Glycosaminoglycans / pharmacology*
  • Heart / drug effects*
  • Isoproterenol / toxicity*
  • Male
  • Molecular Weight
  • Mollusca / chemistry*
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / enzymology
  • Myocardial Infarction / metabolism
  • Myocardial Infarction / pathology
  • Myocardium / enzymology
  • Myocardium / metabolism
  • Myocardium / pathology
  • Oxidative Stress / drug effects*
  • Rats
  • Rats, Wistar
  • Time Factors


  • Antioxidants
  • Glycosaminoglycans
  • Isoproterenol