Modification of a French pressure cell to improve microbial cell disruption

Photosynth Res. 2009 Oct;102(1):95-7. doi: 10.1007/s11120-009-9493-4.


A procedure for modification of the valve stem of a 40 K French pressure cell is described. The modification should be done by a machinist and requires a metalworking lathe. After modification of the valve stem, a torlon 4203 plastic ball is used between the valve stem and valve seat to control the pressure within the cell. The torlon plastic ball is a key component needed to obtain the high pressures required for efficient disruption of microbial cells.

MeSH terms

  • Cell Fractionation / instrumentation*
  • Rhodobacter sphaeroides / cytology*