Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

J Dent. 2009 Dec;37(12):994-8. doi: 10.1016/j.jdent.2009.08.007. Epub 2009 Sep 3.


Objectives: This in situ/ex vivo study aimed to analyse the impact of possible MMP-inhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion.

Methods: Twelve volunteers took part in this cross-over and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoride-free toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60s with: 250 ppm F solution (SnF(2)/AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24, 100% Camellia Sinensis leaf extract, catechin concentration: 30+/-3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (microm). The data were analysed by two-way repeated measures ANOVA and Bonferroni post hoc test.

Results: There was a significant difference between the conditions (EroxEro+Abr, p<0.001) and among the solutions (p<0.001). All solutions (F: 1.42+/-0.34; 1.73+/-0.50, chlorhexidine: 1.15+/-0.26; 1.59+/-0.32, green tea: 1.06+/-0.30; 1.54+/-0.55) significantly reduced the dentin wear when compared to control (2.00+/-0.55; 2.41+/-0.83) for both conditions. There were not significant differences among green tea extract, chlorhexidine and F solutions.

Conclusions: Thus, the possible MMP-inhibitors tested in this study seem to be a promising preventive measure to reduce dentin erosion-abrasion, but their mechanism of action needs to be investigated in further studies.

Publication types

  • Comparative Study
  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amines / therapeutic use
  • Animals
  • Camellia sinensis*
  • Carbonated Beverages / adverse effects
  • Catechin / therapeutic use
  • Cattle
  • Chlorhexidine / therapeutic use*
  • Cross-Over Studies
  • Dentin / drug effects
  • Double-Blind Method
  • Drug Combinations
  • Enzyme Inhibitors / therapeutic use*
  • Female
  • Humans
  • Male
  • Matrix Metalloproteinase Inhibitors
  • Mouthwashes / therapeutic use
  • Plant Extracts / therapeutic use*
  • Prospective Studies
  • Tin Fluorides / therapeutic use
  • Tooth Abrasion / prevention & control*
  • Tooth Erosion / prevention & control*
  • Toothbrushing / adverse effects
  • Young Adult


  • Amines
  • Drug Combinations
  • Enzyme Inhibitors
  • Matrix Metalloproteinase Inhibitors
  • Mouthwashes
  • Plant Extracts
  • Tin Fluorides
  • Meridol
  • Catechin
  • Chlorhexidine