Isolation and characterization of doxorubicin-resistant Lewis lung carcinoma variants

Cancer Commun. 1990;2(4):151-8. doi: 10.3727/095535490820874542.

Abstract

A series of drug-resistant variants of the murine Lewis lung carcinoma (3LL-CK) cell line has been isolated using stepwise selection in increasing concentrations of doxorubicin. These variants exhibited classical multidrug resistance as evidenced by decreased doxorubicin accumulation, cross-resistance to vinblastine, reversibility of resistance by verapamil, and overexpression of P-glycoprotein. When the doxorubicin-resistant 3LL-CK cell populations were injected into the tail veins of B6D2F1 mice, their metastatic abilities were consistently reduced compared with that of the parental line.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Animals
  • Cell Division / drug effects
  • Cell Line
  • Doxorubicin / metabolism
  • Doxorubicin / pharmacology*
  • Drug Resistance*
  • Genetic Variation
  • Lung Neoplasms / metabolism
  • Membrane Glycoproteins / biosynthesis
  • Mice
  • Neoplasm Proteins / biosynthesis
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects*
  • Tumor Cells, Cultured / metabolism
  • Verapamil / pharmacology
  • Vinblastine / pharmacology

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • Vinblastine
  • Doxorubicin
  • Verapamil