Abstract
8-Oxo-2'-deoxyguanosine (8-oxodG) is one of the most important oxidative DNA lesions, and G-rich telomeric DNA is especially susceptible to oxidative DNA damage. RecQ helicases WRN and BLM and telomere-binding protein POT1 are thought to play roles in telomere maintenance. This study examines the ability of WRN, BLM, and RecQ5 to unwind and POT1 to bind telomeric D-loops containing 8-oxodG. The results demonstrate that WRN and BLM preferentially unwind telomeric D-loops containing 8-oxodG and that POT1 binds with higher affinity to telomeric D-loops with 8-oxodG but shows no preference for telomeric single-stranded DNA with 8-oxodG. We speculate that telomeric D-loops with 8-oxodG may have a greater tendency to form G-quadruplex DNA structures than telomeric DNA lacking 8-oxodG.
Publication types
-
Research Support, N.I.H., Intramural
MeSH terms
-
8-Hydroxy-2'-Deoxyguanosine
-
Animals
-
Base Sequence
-
Cell Line
-
Deoxyguanosine / analogs & derivatives*
-
Deoxyguanosine / metabolism
-
Exodeoxyribonucleases / chemistry
-
Exodeoxyribonucleases / genetics
-
Exodeoxyribonucleases / metabolism*
-
Humans
-
Molecular Sequence Data
-
Protein Binding
-
RecQ Helicases / chemistry
-
RecQ Helicases / genetics
-
RecQ Helicases / metabolism*
-
Shelterin Complex
-
Substrate Specificity
-
Telomere / chemistry*
-
Telomere / genetics
-
Telomere / metabolism
-
Telomere-Binding Proteins / chemistry
-
Telomere-Binding Proteins / genetics
-
Telomere-Binding Proteins / metabolism*
-
Werner Syndrome Helicase
Substances
-
POT1 protein, human
-
Shelterin Complex
-
Telomere-Binding Proteins
-
8-Hydroxy-2'-Deoxyguanosine
-
Exodeoxyribonucleases
-
Bloom syndrome protein
-
RecQ Helicases
-
WRN protein, human
-
Werner Syndrome Helicase
-
Deoxyguanosine