Salmonellae of serotypes gallinarum and pullorum grouped by biotyping and fimbrial-gene probing

J Med Microbiol. 1990 Jul;32(3):145-52. doi: 10.1099/00222615-32-3-145.

Abstract

When salmonellae of serotypes Gallinarum (50 isolates) and Pullorum (36 isolates), that produce non-adhesive (type-2) fimbriae, were tested for their reactions in biochemical tests, 81 (94%) were found to belong to three distinct biochemical groups, I-III. Interaction of HinfI-digested DNA of both Gallinarum and Pullorum with a probe of accessory genes of type-1 fimbriation in serotype Typhimurium gave one type of Southern hybridisation pattern that was readily distinguished from that of Typhimurium strains. With a probe of the Typhimurium fimbrial subunit gene, Pullorum isolates were separated into strongly and weakly probe-reactive groups which showed restriction fragment-length polymorphism; these latter groups corresponded to biochemical groups II and III, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Biological Evolution
  • Blotting, Southern
  • DNA Probes
  • DNA, Bacterial / analysis
  • Fermentation
  • Fimbriae, Bacterial / analysis
  • Polymorphism, Restriction Fragment Length
  • Salmonella / classification*
  • Salmonella / metabolism

Substances

  • DNA Probes
  • DNA, Bacterial