Mechanisms of polycomb gene silencing: knowns and unknowns

Nat Rev Mol Cell Biol. 2009 Oct;10(10):697-708. doi: 10.1038/nrm2763. Epub 2009 Sep 9.


Polycomb proteins form chromatin-modifying complexes that implement transcriptional silencing in higher eukaryotes. Hundreds of genes are silenced by Polycomb proteins, including dozens of genes that encode crucial developmental regulators in organisms ranging from plants to humans. Two main families of complexes, called Polycomb repressive complex 1 (PRC1) and PRC2, are targeted to repressed regions. Recent studies have advanced our understanding of these complexes, including their potential mechanisms of gene silencing, the roles of chromatin modifications, their means of delivery to target genes and the functional distinctions among variant complexes. Emerging concepts include the existence of a Polycomb barrier to transcription elongation and the involvement of non-coding RNAs in the targeting of Polycomb complexes. These findings have an impact on the epigenetic programming of gene expression in many biological systems.

Publication types

  • Review

MeSH terms

  • Animals
  • Chromatin / metabolism
  • DNA Methylation
  • Epigenesis, Genetic
  • Gene Expression Regulation
  • Gene Silencing*
  • Genes, Homeobox*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Humans
  • Models, Biological
  • Polycomb-Group Proteins
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*


  • Chromatin
  • Homeodomain Proteins
  • Polycomb-Group Proteins
  • Repressor Proteins