Expansion of FOXP3-positive CD4+CD25+ T cells associated with disease activity in atopic dermatitis

Ann Allergy Asthma Immunol. 2009 Aug;103(2):160-5. doi: 10.1016/S1081-1206(10)60170-6.


Background: FOXP3-positive CD4+CD25+ T cells are known to have an immunoregulatory function by means of preventing T-cell reactivity to both self- and non-self-antigens. However, the role of these cells in the pathogenesis of allergic diseases is not clear.

Objective: To evaluate the quantity and quality of circulating FOXP3-positive T cells in patients with atopic dermatitis (AD).

Methods: Peripheral blood mononuclear cells were isolated from 35 AD patients (mean [SD] age, 27.1 [7.5] years) and 36 controls (mean [SD] age, 27.5 [10.0] years). Cellular FOXP3 expression was analyzed using flow cytometry. Characteristics of FOXP3-positive T cells were evaluated with respect to cytokine production capability and suppressive function.

Results: Frequencies of circulating FOXP3+CD25+ cells in the CD4+ T-cell population of AD patients were significantly higher than those in controls (mean [SD], 7.4% [4.6%] vs 4.5% [1.3%]; P = .002) and correlated with their Scoring Atopic Dermatitis (SCORAD) scores (r = 0.74, P = .008) and peripheral blood eosinophil counts (r = 0.72, P < .001). In the patients whose samples were analyzed at intervals of 1 to 2 months, frequencies of FOXP3-positive T cells were decreased as their skin lesions improved, regardless of medicines used. FOXP3-positive CD4+ T cells from patients, as well as those from controls, showed little capability to synthesize interferon gamma and interleukin 4. No differences were found in suppression abilities of CD4+CD25+ T cells between AD patients and controls.

Conclusions: Our data suggest that dynamic fluctuation in numbers of circulating FOXP3-positive regulatory T cells might contribute to the pathogenesis of AD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • CD4 Lymphocyte Count
  • Coculture Techniques
  • Dermatitis, Atopic / diagnosis
  • Dermatitis, Atopic / drug therapy
  • Dermatitis, Atopic / etiology
  • Dermatitis, Atopic / immunology*
  • Eosinophils / cytology
  • Female
  • Forkhead Transcription Factors / metabolism*
  • Humans
  • Immune Tolerance / immunology
  • Immunoglobulin E / blood
  • Interferon-gamma / metabolism
  • Interleukin-2 Receptor alpha Subunit / metabolism*
  • Interleukin-4 / metabolism
  • Leukocyte Count
  • Male
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism
  • T-Lymphocytes, Regulatory / cytology
  • T-Lymphocytes, Regulatory / immunology*
  • T-Lymphocytes, Regulatory / metabolism
  • Young Adult


  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • IL2RA protein, human
  • IL4 protein, human
  • Interleukin-2 Receptor alpha Subunit
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma