Abstract
A method for the synthesis of small molecule macroarrays of N-acylated L-homoserine lactones (AHLs) is reported. A focused library of AHLs was constructed, and the macroarray platform was found to be compatible with both solution and agar-overlay assays using quorum-sensing (QS) reporter strains. Several QS antagonists were discovered and serve to showcase the macroarray as a straightforward technique for QS research.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Butyrolactone / analogs & derivatives*
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4-Butyrolactone / chemical synthesis
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4-Butyrolactone / chemistry
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4-Butyrolactone / pharmacology
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Acylation
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Aliivibrio fischeri / cytology
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Aliivibrio fischeri / drug effects
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Chromobacterium / cytology
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Chromobacterium / drug effects
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Cyclization
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Nitrogen / chemistry
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Quorum Sensing / drug effects*
Substances
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homoserine lactone
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Nitrogen
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4-Butyrolactone