Determination of imatinib plasma levels in patients with chronic myeloid leukemia by high performance liquid chromatography-ultraviolet detection and liquid chromatography-tandem mass spectrometry: methods' comparison

Leuk Res. 2010 Jun;34(6):714-7. doi: 10.1016/j.leukres.2009.08.005. Epub 2009 Sep 9.

Abstract

The aims of this study were to validate and compare HPLC and LCMSMS analytical methods and their applicability for the quantitation of imatinib in human plasma. A total of 50 patients with chronic myeloid leukemia (CML) in chronic phase (CP) receiving 400 mg/day imatinib were enrolled in the study. Drug levels were determined by HPLC-UV and LCMSMS. HPLC intra-day accuracy ranged from 100.51 to 103.19%. LCMSMS accuracy ranged from 89.72 to 106.29%. The correlation coefficient between both methods was r(2)=0.96. HPLC can be used for imatinib levels' determinations in patients accurately and precisely.

Publication types

  • Comparative Study
  • Validation Study

MeSH terms

  • Adult
  • Antineoplastic Agents / analysis
  • Antineoplastic Agents / blood
  • Antineoplastic Agents / therapeutic use
  • Benzamides
  • Blood Chemical Analysis / methods*
  • Blood Chemical Analysis / standards
  • Calibration
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, High Pressure Liquid / standards
  • Chromatography, Liquid / methods
  • Chromatography, Liquid / standards
  • Circadian Rhythm / physiology
  • Female
  • Humans
  • Imatinib Mesylate
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / blood*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy
  • Male
  • Middle Aged
  • Piperazines / analysis*
  • Piperazines / blood
  • Piperazines / therapeutic use
  • Protein Kinase Inhibitors / analysis
  • Protein Kinase Inhibitors / blood
  • Protein Kinase Inhibitors / therapeutic use
  • Pyrimidines / analysis*
  • Pyrimidines / blood
  • Pyrimidines / therapeutic use
  • Randomized Controlled Trials as Topic
  • Sensitivity and Specificity
  • Spectrophotometry, Ultraviolet / methods
  • Spectrophotometry, Ultraviolet / standards
  • Tandem Mass Spectrometry / methods*
  • Tandem Mass Spectrometry / standards

Substances

  • Antineoplastic Agents
  • Benzamides
  • Piperazines
  • Protein Kinase Inhibitors
  • Pyrimidines
  • Imatinib Mesylate