Role of Cybr, a cytohesin binder and regulator, in CD4(+) T-cell function and host immunity

Mol Immunol. 2009 Oct;46(16):3218-23. doi: 10.1016/j.molimm.2009.08.014. Epub 2009 Sep 9.


Cytohesin binder and regulator (Cybr) is known to regulate leukocyte adhesion and migration. However, its function in T-cells is poorly understood. Here, we investigated the role of Cybr in CD4(+) T-cell function and host immunity. Cybr inhibited p38 phosphorylation following CD4(+) T-cell stimulation. Since p38 regulates the expression of T-box expressed in T-cells (T-bet) but not GATA binding protein 3 (GATA-3) in T-cells, Cybr decreased the expression of T-bet and IFN-gamma in CD4(+) T-cells. Moreover, we found that host immunity against Listeria infection and IFN-gamma production in blood were significantly compromised in Cybr-overexpressing transgenic mice. In summary, our data suggest that Cybr represses the expression of T-bet and IFN-gamma via an inhibition of p38 in T-cells and consequently reduces host resistance to bacterial infection in mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / metabolism
  • Carrier Proteins / immunology*
  • Carrier Proteins / metabolism
  • GATA3 Transcription Factor / biosynthesis
  • GATA3 Transcription Factor / immunology
  • Gene Expression Regulation / immunology*
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / immunology*
  • Listeria monocytogenes / immunology*
  • Listeriosis / blood
  • Listeriosis / immunology*
  • Membrane Proteins / immunology*
  • Membrane Proteins / metabolism
  • Mice
  • Phosphorylation / immunology
  • T-Box Domain Proteins / biosynthesis
  • T-Box Domain Proteins / immunology
  • p38 Mitogen-Activated Protein Kinases / immunology
  • p38 Mitogen-Activated Protein Kinases / metabolism


  • Carrier Proteins
  • Cybr protein, mouse
  • GATA3 Transcription Factor
  • Gata3 protein, mouse
  • Membrane Proteins
  • T-Box Domain Proteins
  • T-box transcription factor TBX21
  • Interferon-gamma
  • p38 Mitogen-Activated Protein Kinases