The relationship between colony-forming ability and chromosomal aberrations induced in human T-lymphocytes after gamma-irradiation

Int J Radiat Biol. 1990 Aug;58(2):293-301. doi: 10.1080/09553009014551631.


Using aliquots from the same samples of irradiated normal human blood, T-lymphocyte survival was measured by (a) 14-day colony growth, (b) the proportion of chromosomally normal first post-irradiation metaphases and (c) the proportion of cells not suffering interphase death and delay within 48 h after irradiation. Combining (b) and (c) gives a prediction of the proportion of cytogenetically undamaged cells which reach metaphase after 48 h. These cells should be capable of producing viable colonies under suitable culture conditions. A comparison of these values with those observed in (a) shows a reasonable agreement in accordance with the hypothesis that gross chromosomal damage and cell reproductive death are closely related.

MeSH terms

  • Cell Survival / radiation effects
  • Chromosome Aberrations*
  • Gamma Rays
  • Humans
  • Male
  • T-Lymphocytes / cytology
  • T-Lymphocytes / radiation effects*