Regenerative Effects of Basic Fibroblast Growth Factor on Extracellular Matrix Production in Aged Rat Vocal Folds

Ann Otol Rhinol Laryngol. 2009 Aug;118(8):559-64. doi: 10.1177/000348940911800805.

Abstract

Objectives: We investigated acute changes in extracellular matrix (ECM) gene expression and histologic changes in the deposition of collagen and hyaluronan (hyaluronic acid; HA) after basic fibroblast growth factor (bFGF) treatment of the aged rat vocal fold.

Methods: For the polymerase chain reaction (PCR) experiments, we divided ten 18-month-old Sprague-Dawley rats into two groups that received serial injections of sham (saline solution) or bFGF (2 ng/microL) and euthanized them 2 weeks after the initial injection to investigate acute changes in ECM gene expression. We treated a separate group of 5 animals unilaterally and sacrificed them 4 weeks after the initial injection to investigate histologic changes in the deposition of collagen and HA.

Results: Real-time PCR revealed significantly up-regulated HA synthase (HAS)-2, HAS-3, matrix metalloproteinase (MMP)-2, and procollagen type I gene expression in the bFGF treatment group as compared to the sham treatment group. Histologic staining revealed significantly increased deposition of HA in the bFGF-treated vocal fold as compared to the sham-treated vocal fold. No differences in ECM collagen levels were observed between treatment sides.

Conclusions: Basic fibroblast growth factor induced the up-regulation of HAS-2, HAS-3, MMP-2, and procollagen type I. Histologically, aged vocal folds treated with bFGF revealed increased deposition of HA as compared to sham-treated vocal folds.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aging / physiology*
  • Animals
  • Collagen Type I / genetics
  • Collagen Type I / metabolism
  • Extracellular Matrix / drug effects*
  • Extracellular Matrix / pathology
  • Extracellular Matrix / physiology*
  • Fibroblast Growth Factor 2 / pharmacology*
  • Glucuronosyltransferase / genetics
  • Glucuronosyltransferase / metabolism
  • Hyaluronan Synthases
  • Male
  • Matrix Metalloproteinase 2 / genetics
  • Matrix Metalloproteinase 2 / metabolism
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism
  • Procollagen / genetics
  • Procollagen / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Regeneration / drug effects*
  • Vocal Cords / drug effects*
  • Vocal Cords / metabolism
  • Vocal Cords / pathology

Substances

  • Collagen Type I
  • Procollagen
  • RNA, Messenger
  • Fibroblast Growth Factor 2
  • Glucuronosyltransferase
  • Has2 protein, rat
  • Has3 protein, rat
  • Hyaluronan Synthases
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9