Natural killer cells are polarized toward cytotoxicity in chronic hepatitis C in an interferon-alfa-dependent manner

Gastroenterology. 2010 Jan;138(1):325-35.e1-2. doi: 10.1053/j.gastro.2009.08.066. Epub 2009 Sep 10.


Background & aims: Patients with chronic hepatitis C virus (HCV) infection display great variability in disease activity and progression. Although virus-specific adaptive immune responses have been characterized extensively and found to be impaired in chronic hepatitis C, the role of innate immune responses in disease activity and progression of chronic hepatitis C is not well understood.

Methods: We studied 42 HCV-infected patients and 12 healthy uninfected controls.

Results: We found an increased frequency of natural killer (NK) cells expressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), NKp44, NKG2C, and CD122 in chronic hepatitis C as compared with healthy controls (P < .05 for all markers) and stronger activation of NK cells in the liver than in the blood (P < .05). This NK cell phenotype was associated with polarization of NK cell function toward CD107a expression as a marker of degranulation, but with not increased interferon (IFN)-gamma production of CD56(dim) NK cells. The polarized NK cell phenotype correlated with alanine aminotransferase levels (r(2) = 0.312, P = .03). To investigate whether in vivo exposure of NK cells to HCV-induced type I IFN was causing this NK cell phenotype, peripheral blood mononuclear cells from 10 healthy controls and 8 HCV-infected patients were stimulated in the presence of IFN-alfa, which resulted in increased NK cell expression of TRAIL and CD107a (P < .001), but not IFN-gamma.

Conclusions: Collectively, these results describe a polarized NK cell phenotype induced by chronic exposure to HCV-induced IFN-alfa. This phenotype may contribute to liver injury through TRAIL expression and cytotoxicity, whereas the lacking increase in IFN-gamma production may facilitate the inability to clear HCV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Degranulation / drug effects
  • Cell Degranulation / immunology
  • Cell Polarity / immunology
  • Cells, Cultured
  • Disease Progression
  • Flow Cytometry
  • Hepatitis C, Chronic / immunology*
  • Humans
  • Immunophenotyping
  • Interferon-alpha / immunology
  • Interferon-alpha / pharmacology*
  • Interferon-gamma / metabolism
  • Interleukin-2 Receptor beta Subunit / metabolism
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / metabolism*
  • Killer Cells, Natural / virology*
  • Liver / immunology
  • Liver / virology
  • NK Cell Lectin-Like Receptor Subfamily C / metabolism
  • Natural Cytotoxicity Triggering Receptor 2 / metabolism
  • TNF-Related Apoptosis-Inducing Ligand / metabolism


  • Interferon-alpha
  • Interleukin-2 Receptor beta Subunit
  • KLRC2 protein, human
  • NCR2 protein, human
  • NK Cell Lectin-Like Receptor Subfamily C
  • Natural Cytotoxicity Triggering Receptor 2
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Interferon-gamma