DcR3 protects THP-1 macrophages from apoptosis by increasing integrin alpha4

Biochem Biophys Res Commun. 2009 Nov 27;389(4):593-8. doi: 10.1016/j.bbrc.2009.09.027. Epub 2009 Sep 11.


In order to analyze the function of DcR3 for the regulation of cell adhesion and apoptosis in macrophages, we investigated the expression of decoy receptor 3 (DcR3) in THP-1 monocytes/macrophages. DcR3 was expressed in THP-1 and increased by phorbol 12-myristate 13-acetate (PMA). The formation of macrophage aggregates was observed when THP-1 cells were differentiated by PMA or stimulated with DcR3-Fc. Undifferentiated THP-1 cells were also induced to form aggregates by DcR3-Fc. The expression of integrin alpha4 was significantly increased by DcR3-Fc. CHX-induced apoptosis in THP-1 was inhibited by DcR3-Fc, of which inhibition against CHX-induced apoptosis and aggregate formation were ameliorated by anti-VLA4 antibody. DcR3 may play a significant role in macrophages not only by a decoy receptor but also by increasing alpha4 integrin.

MeSH terms

  • Antibodies / immunology
  • Apoptosis*
  • Cell Line
  • Humans
  • Integrin alpha Chains / antagonists & inhibitors
  • Integrin alpha Chains / immunology
  • Integrin alpha4 / biosynthesis*
  • Macrophages / physiology*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Tumor Necrosis Factor, Member 6b / genetics
  • Receptors, Tumor Necrosis Factor, Member 6b / metabolism*


  • Antibodies
  • CDw49d
  • Integrin alpha Chains
  • RNA, Messenger
  • Receptors, Tumor Necrosis Factor, Member 6b
  • TNFRSF6B protein, human
  • Integrin alpha4