Metabolism of stanozolol: chemical synthesis and identification of a major canine urinary metabolite by liquid chromatography-electrospray ionisation ion trap mass spectrometry

J Steroid Biochem Mol Biol. 2009 Nov;117(4-5):152-8. doi: 10.1016/j.jsbmb.2009.09.002. Epub 2009 Sep 11.


The canine phase I and phase II metabolism of the synthetic anabolic-androgenic steroid stanozolol was investigated following intramuscular injection into a male greyhound. The major phase I biotransformation was hydroxylation to give 6alpha-hydroxystanozolol which was excreted as a glucuronide conjugate and was identified by comparison with synthetically derived reference materials. An analytical procedure was developed for the detection of this stanozolol metabolite in canine urine using solid phase extraction, enzyme hydrolysis of glucuronide conjugates and analysis by positive ion electrospray ionisation ion trap LC-MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anabolic Agents / chemical synthesis
  • Anabolic Agents / pharmacokinetics*
  • Anabolic Agents / urine
  • Androgens / chemical synthesis
  • Androgens / pharmacokinetics*
  • Androgens / urine
  • Animals
  • Chromatography, Liquid / methods*
  • Crystallography, X-Ray
  • Dogs
  • Magnetic Resonance Spectroscopy
  • Male
  • Models, Molecular
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Stanozolol / chemical synthesis
  • Stanozolol / pharmacokinetics*
  • Stanozolol / urine


  • Anabolic Agents
  • Androgens
  • Stanozolol